[Molecular identification of mycoplasma agents in suspected contagious caprine pleuropneumonia]

Contagious Caprine Pleuropneumonia [CCPP] is one of the common infections in the middle east regions. So far, there has not been received any report about isolation and identification of these agents in Iran. The aim of this study is to diagnose and isolate mycoplasma agents in suspected goat flocks. Total of 100 pneumonic lung specimen from 20 CCPPsuspected flocks were collected from abbatoirs close to Kermanshah during 1384-1386 and had been sent to Microbiology Lab. Gross lesions showed hepatization with grey and white lesions [consolidation] and motley appearance with or without fibrin. The minced tissue were inoculated to PPLO broth agar. After multiple passages, typical mycoplasma colony was isolated from 4 flocks [22/2%]. Mycoplasma DNA was also extracted based on phenolchloroform method and subjected to generic PCR with specific primers. In addition to the perivious positive samples from tissue culture, 5other flocks also showed contamination with Mycoplasma organisms in PCR tests[45%]. Then, the samples were determined for Mycoplasma mycoides cluster infection, M. capricolum capripneumonia and M. mycoides mycoides [L.C], using M. agalactia as negative control, with specific primers in PCR, there has showed no contamination to these strains. However, to declare "free status " from CCPP in goat flocks requires more developed researches and much more samples in further investigation

[ study on the presence of Escherichia coli, Coccidia and Cryptosporidium in stool samples of under one month age diarrheic calves in Ghaernshahr and Babol and antibiotic sensitivity of isolates]

Objective: study on the presence of Escherichia coli, Coccidia and Cryptosporidium in stool samples of neonatal diarrheic calves in Ghaemshahr and Babol, simultaneous shedding of Coccidia and Cvyptosporidium with Escherichia coli in these calves, serotyping of Escherichia coli, comparison of antibiotic sensitivity of K99+ and other serotypes of Escherichia coli

Animals: ninety three diarrheic neonatal calves [under one month]

Procedure: taking stool sample from rectum of the diarrheic calves, using standard methods for detection of Coccidia and Cryptosporidium, isolation of Escherichia coli canied out by using standard bacteriological methods and serotyping and antibiotic sensitivity test of isolates

Statistical analysis: results were reported by descriptive scales

Results: Escherichia coli were isolated from 40.8% of diarrheic calves from which only one isolate were K99+ [1.07%]. In 12 samples two pathogens have been diagnosed simultaneously which in 6 samples E. coli and Coccidia [6.4%] and 6 samples E. coli and Cryptosporidium [6.4%] have been isolated. Isolated Escherichia coli were resistant to many antibiotics which routinely used in treatment of diarrhea and there was no significant difference between K99+ and other isolates of Escherichia coli in antibiotic sensitivity test

Clinical implications: from the results of this study it seems that K99+ Escherichia coli is not a common isolate in neonatal diarrheic calves in Ghaemshahr and Babol